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KMID : 1149220180260030183
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Korean Journal of Oriental Medical Prescription
2018 Volume.26 No. 3 p.183 ~ p.193
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Epimedium koreanum Nakai Water Extract Regulates Hepatic Stellate Cells Activation through Inhibition of Smad Signaling Pathway
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Jung Ji-Yun
Min Byung-Gu
Park Chung-A
Byun Sung-Hui
Cho Il-Je
Kim Sang-Chan
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Abstract
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Objectives : In Traditional Korean Medicine, Epimedium koreanum Nakai has diverse pharmacological activities to treat impotence, forgetfulness, cataract and exophthalmos. Present study investigated anti-fibrogenic effects of E. koreanum water extract (EKE) in hepatic stellate cells (HSCs).
Methods : To study anti-fibrogenic effects of EKE, LX-2 cells, a human immortalized HSCs, were pre-treated with 3-300 μg/mL of EKE, and then subsequently exposed to 5 ng/mL of transforming growth factor-β1 (TGF-β1). Expression level of α-smooth muscle actin was determined by immunoblot analysis. Phosphorylation of Smad, transactivation of Smad, and expression of plasminogen activator inhibitor-1 (PAI-1) were monitored to investigate the effect of EKE on TGF-β1-mediated signaling pathway.
Results : Up to 100 μg/mL, EKE did not show any cytotoxicity on LX-2 cells. Pre-treatment of EKE (100 μg/mL) significantly inhibited α-smooth muscle actin expression induced by TGF-β1. In addition, EKE significantly decreased Smad2 and Smad3 phosphorylations, Smad binding element-driven luciferase activity and PAI-1 expression by TGF-β1. Of three flavonoid compounds found in EKE, only quercertin (30 μM) attenuated TGF-β1-mediated PAI-1 expression.
Conclusion : These results suggest that EKE has an ability to suppress fibrogenic process in HSCs via inhibition of TGF-β1/Smad signaling pathway.
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KEYWORD
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Epimedium koreanum Nakai, Hepatic stellate cells (HSCs), Transforming growth factor-β1 (TGF-β1), Smad, Quercetin
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